Dedifferentiated Schwann Cell Precursors Secreting Paracrine Factors Are Required for Regeneration of the Mammalian Digit Tip
| Autor: | David R. Kaplan, Scott A. Yuzwa, Smitha Paul, Freda D. Miller, Matthew J. Carr, Matthew P. Krause, Mekayla Storer, Adam P. W. Johnston, Neemat Mahmud, Karen Louise Jones |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Growth factor medicine.medical_treatment Regeneration (biology) Mesenchymal stem cell Schwann cell Cell Biology Biology Cell biology Transplantation 03 medical and health sciences Paracrine signalling 030104 developmental biology medicine.anatomical_structure Immunology Genetics medicine Molecular Medicine Bone regeneration Blastema |
| Zdroj: | Cell Stem Cell. 19:433-448 |
| ISSN: | 1934-5909 |
| Popis: | Adult mammals have lost multi-tissue regenerative capacity, except for the distal digit, which is able to regenerate via mechanisms that remain largely unknown. Here, we show that, after adult mouse distal digit removal, nerve-associated Schwann cell precursors (SCPs) dedifferentiate and secrete growth factors that promote expansion of the blastema and digit regeneration. When SCPs were dysregulated or ablated, mesenchymal precursor proliferation in the blastema was decreased and nail and bone regeneration were impaired. Transplantation of exogenous SCPs rescued these regeneration defects. We found that SCPs secrete factors that promote self-renewal of mesenchymal precursors, and we used transcriptomic and proteomic analysis to define candidate factors. Two of these, oncostatin M (OSM) and platelet-derived growth factor AA (PDGF-AA), are made by SCPs in the regenerating digit and rescued the deficits in regeneration caused by loss of SCPs. As all peripheral tissues contain nerves, these results could have broad implications for mammalian tissue repair and regeneration. |
| Databáze: | OpenAIRE |
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