Screening antioxidant capacity of Mexican maize (Zea mays L.) landraces with colored grain using ABTS, DPPH and FRAP methods

Autor: Ma. Teresa Sumaya-Martínez, Orlando Ramírez-García, Alberto Santillán-Fernández, Yolanda Salinas-Moreno
Rok vydání: 2021
Předmět:
Zdroj: Cereal Research Communications. 50:1075-1083
ISSN: 1788-9170
0133-3720
DOI: 10.1007/s42976-021-00221-6
Popis: The antioxidant capacity (AC) of pigmented cereal grains is associated with their nutraceutical value. The AC of grain from a diverse panel of maize genotypes with phenolic pigments was investigated. Our aims were to measure the AC of maize accessions with blue purple and red grain using the ABTS, DPPH and FRAP methods, and to determine which method was most sensitive to variation in phenolic grain compounds. Grain from 116 maize landraces with blue purple (BPMG), cherry red (CRMG) and brick red grain (BRMG) colors was used. Total soluble phenolics (TSP), total anthocyanin content (TAC), and proanthocyanidins (PAs) were determined. The TSP fraction was used to evaluate the AC of the maize accessions. The AC in BPMG and CRMG was highly related to TAC, whereas in BRMG it was associated with TSP. The highest variation in AC in each maize grain color was observed using the ABTS method, and the least variation was obtained by the DPPH method. The color of maize grain did not determine its AC, since samples with high AC were identified in all three groups. The most sensitive methods to detect variation in TAC with BPMG and CRMG maize were ABTS and FRAP, whereas for BRMG, the FRAP method was the most sensitive to variation in TSP.
Databáze: OpenAIRE