Expression of GM-CSF receptors in male germ cells and their role in signaling for increased glucose and vitamin C transport

Autor: Monica Brito, Angara Zambrano, Ilona I. Concha, Juan C. Slebe, Enrique Werner, M. Cecilia Rauch, Rodolfo Amthauer, Carla Noli, Juan Carlos Vera
Rok vydání: 2001
Předmět:
Zdroj: Journal of Cellular Biochemistry. 80:625-634
ISSN: 1097-4644
0730-2312
DOI: 10.1002/1097-4644(20010315)80:4<625::aid-jcb1017>3.0.co;2-9
Popis: We studied the expression and function of the granulocyte-macrophage colony stimulating factor (GM-CSF) receptor in male germ cells. RT-PCR showed expression of mRNAs encoding the α- and β-subunits of the GM-CSF receptor in human testis, and the presence of the α- and β-proteins was confirmed by immunoblotting with anti-α and anti-β-antibodies. Immunolocalization studies showed the level of expression of GM-CSF α- and β-subunits in the germ line in the testis and in ejaculated spermatozoa. Receptor binding studies using radiolabeled GM-CSF revealed that bull spermatozoa have about 105 high-affinity sites with a Kd of 222 pM and ≈1100 low-affinity sites with a Kd of 10 nM. GM-CSF signaled, in a time- and dose-dependent manner, for an increased uptake of glucose and vitamin C. J. Cell. Biochem. 80:625–634, 2001. © 2001 Wiley-Liss, Inc.
Databáze: OpenAIRE
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