Isocratic high-performance liquid chromatographic determination of plasma adenosine

Autor: E. Huszar, M. Kollai, E. Barat
Rok vydání: 1996
Předmět:
Zdroj: Chromatographia. 42:318-322
ISSN: 1612-1112
0009-5893
DOI: 10.1007/bf02290317
Popis: Due to manifold physiological and cardioprotective actions of adenosine, the demand for a simple but accurate method to determine its concentration in plasma is increasing. The aim of this study was firstly to develop a simple isocratic method instead of the gradient elution or peak-shifting techniques used earlier and secondly to check conflicting data on the composition of “stop-solution”, added to the sample in order to prevent changes in adenosine concentration. Isocratic elution improved signal to noise ratio and concentrations of 100 μmol L−1 dipyridamole and 2.5 μmol L−1 erythro-9(2-hydroxy-3-nonyl)adenine in the blood sample effectively prevented both adenosine formation and degradation, even without the use of a 5′-ecto-nucleotidase inhibitor. Lowering the concentration of dipyridamole to 25 μmol L−1 caused more than a tenfold increase of adenosine concentration in two out of five cases and even 100 μmol L−1 dipyridamole alone is not sufficient to inhibit adenosine deaminase in blood samples.
Databáze: OpenAIRE
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