Memristive biosensors based on full-size antibodies and antibody fragments
| Autor: | Sandro Carrara, Richard O'Kennedy, Sebastien Naus, Ioulia Tzouvadaki, Giovanni De Micheli, Julia Zapatero-Rodríguez |
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| Rok vydání: | 2019 |
| Předmět: |
Materials science
biology Metals and Alloys Nanowire Nanotechnology 02 engineering and technology Free prostate specific antigen 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences Antibody fragments 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Antigen Materials Chemistry biology.protein Electrical and Electronic Engineering Antibody 0210 nano-technology Silicon nanowires Instrumentation Biosensor Single-Chain Antibodies |
| Zdroj: | Sensors and Actuators B: Chemical. 286:346-352 |
| ISSN: | 0925-4005 |
| Popis: | For the first time memristive biosensors developed by the incorporation of scAbs (single-chain antibody fragments) enabling label-free detection of free Prostate Specific Antigen (fPSA) for early-stage detection of prostate cancer (PC) are reported. In addition, the first ever-published report of a direct relationship between the size of the bio-probes used to transfer specificity to the sensor and the voltage gap is here included. The proposed bio-detection is achieved by means of a voltage gap acquired in the electrical response of memristive sensors obtained by the bio-modification of silicon nanowire arrays. A comparison between memristive biosensors based on full-size antibodies and antibody fragments is also demonstrated by analysis of the surface morphology via Scanning Electron Microscopy. This new kind of biosensors provide excellent analytical sensitivity with a limit-of-detection down to 0.144 pg/mL on PSA. |
| Databáze: | OpenAIRE |
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