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Three species of mycobateria are shown to afford quite a high level of insoluble, non-hydrolysable (INH) material when subjected to usual drastic saponification and acid hydrolysis. The spectroscopic features (FTIR, solid-state 13C NMR), the thermal behaviour (DTG analysis) and the chemical degradation products (alkaline KMnO4 oxidation) of these materials are compared with those of melanoidin-like polymers synthesized from a mixture of monosaccharides and amino acids occurring in the mycobacterial cell walls. The mycobacterial INH materials, thus, appear to correspond to artifacts generated during the isolation process. The implication of these results, on the contribution of such melanoidin-like artifact macromolecular materials in the INH residues previously isolated from other microorganisms is discussed. A new process of isolation avoiding the formation of such artifacts is proposed. |
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