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The objective of this study was to compare the kinetic characteristics of some key components of monoclonal antibody synthesis (growth, cell specific productivity, mRNAs coding for heavy and light chain IgG) in hybridoma continuous cultures in a protein-free medium, submitted to various dilution rates (0.0156–0.0523/h), with data obtained from semicontinuous cultures. As for the semicontinuous cultures there was an increase in the specific growth rate, viability, the levels of mRNAs coding for heavy and light chains of IgG and glucose consumption and lactate production rates with increasing dilution rates. The maximum viable cell density of about 1.8×106 c/ml was obtained at a dilution rate of 0.036/h. The main differences in comparison to semicontinuous cultures were the maximal values obtained, in particular, for the antibody concentration and the specific antibody productivity, which were 5 to 10 times higher in the continuous culture. The data were compared to batch cultures, by using the same media as for the semicontinuous and the continuous cultures, in order to establish the effect of both media on growth, production, and the mRNA kinetics for this hybridoma cell 1ine. Generally, it could be confirmed that the regulation of antibody-synthesis and -production is on a posttranslational level and that at higher growth rates more biosynthetic activity is diverted to biomass production. |
