Optimization of chitinase production by a new Streptomyces griseorubens C9 isolate using response surface methodology
| Autor: | Kitouni Mahmoud, Gasmi Meriem |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Plackett–Burman design Liquid culture Chitinase activity Factorial experiment Biology 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences 030104 developmental biology 010608 biotechnology Botany Chitinase biology.protein Yeast extract Response surface methodology Food science Streptomyces griseorubens |
| Zdroj: | Annals of Microbiology. 67:175-183 |
| ISSN: | 1869-2044 1590-4261 |
| Popis: | The purpose of this article is to use statistical Plackett–Burman and Box–Wilson response surface methodology to optimize the medium components and, thus, improve chitinase production by Streptomyces griseorubens C9. This strain was previously isolated and identified from a semi-arid soil of Laghouat region (Algeria). First, syrup of date, colloidal chitin, yeast extract and K2HPO4, KH2PO4 were proved to have significant effects on chitinase activity using the Plackett–Burman design. Then, an optimal medium was obtained by a Box–Wilson factorial design of response surface methodology in liquid culture. Maximum chitinase production was predicted in medium containing 2% colloidal chitin, 0.47% syrup of date, 0.25 g/l yeast extract and 1.81 g/l K2HPO4, KH2PO4 using response surface plots of the STATISTICA software v.12.0. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink