Fatty acid composition and mRNA expression levels of lipid-metabolic genes in the muscles of ovariectomised young goats
| Autor: | Fang Peng, Lei Zhang, Yan-yan Wang, Lu Wan, Zhan-qin Zhou, Fu Mingzhe, Guang Li |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
chemistry.chemical_classification
medicine.medical_specialty Lipoprotein lipase biology Chemistry Pyruvate carboxylase Fatty acid synthase Endocrinology Biochemistry Internal medicine Saturated fatty acid biology.protein medicine Animal Science and Zoology Intramuscular fat Lipase Gene Food Science Polyunsaturated fatty acid |
| Zdroj: | Animal Production Science. 56:1585 |
| ISSN: | 1836-0939 |
| Popis: | This study investigated the fatty acid composition and mRNA levels of acetyl-coenzyme A carboxylase (ACC), fatty acid synthase (FAS), hormone-sensitive lipase (HSL) and lipoprotein lipase (LPL) genes in the Longissimus dorsi muscle (LD) and Biceps femoris muscle (BF) of nine ovariectomised young goats, 50 days after ovariectomy at 5 months of age, relative to nine Control goats. Ovariectomy resulted in faster growth rates (130 vs 110 g/days; P = 0.027), more intramuscular fat (3.37% vs 1.62%; P = 0.009) and heavier final weights (28.3 vs 26.6 kg; P = 0.035). The total saturated fatty acid, C15:0 in the LD and C6:0, C11:0, C12:0, C15:0 and C21:0 in the BF were lower in ovariectomised goats than that in the Control goats (P < 0.05). Ovariectomy increased the total monounsaturated fatty acids in the LD (P < 0.05) and BF (P < 0.01). In the BF, the total polyunsaturated fatty acids and C18:2 of ovariectomised goats were lower than those of the Control goats (P < 0.05), and the C20:2 content significantly decreased in the LD (P < 0.05). The LD of the ovariectomised goats expressed significantly higher FAS but lower HSL mRNA levels than those of Control goats (P < 0.01). Significantly higher ACC, FAS and LPL and lower HSL mRNA levels were observed in the BF of the ovariectomised goats than in the BF of Controls (P < 0.05). These data suggest that ovariectomy affects fatty acid composition potentially by altering the expression of lipid-metabolic genes in the muscles of young goats. |
| Databáze: | OpenAIRE |
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