Nod2 influences microbial resilience and susceptibility to colitis following antibiotic exposure
| Autor: | S Rouquier, Galliano Zanello, Catherine J. Streutker, Susan J. Robertson, Ashleigh Goethel, Williams Turpin, Kenneth Croitoru, Dana J. Philpott |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.drug_class Immunology Antibiotics Inflammation Gut flora Inflammatory bowel disease 03 medical and health sciences 0302 clinical medicine medicine Genetic predisposition Immunology and Allergy Weaning Colitis biology business.industry Amoxicillin medicine.disease biology.organism_classification digestive system diseases 030104 developmental biology medicine.symptom business 030215 immunology medicine.drug |
| Zdroj: | Mucosal Immunology. 12:720-732 |
| ISSN: | 1933-0219 |
| Popis: | Inflammatory bowel disease (IBD) etiology involves genetic susceptibility, environmental triggers, and the gut microbiome. Antibiotic exposure is associated with IBD, both in early life and adulthood. Here, we investigated whether Nod2-deficiency influenced response of the gut microbiota to antibiotics and subsequent colitis susceptibility. Wild-type and Nod2-/- littermate mice were treated with amoxicillin as adults or neonates, and fecal samples were collected for 16S rRNA sequencing. Five weeks after antibiotic exposure, dextran sulfate sodium (DSS) colitis was induced. Antibiotic treatment altered the microbiota of adult WT and Nod2-/- mice, but recovery was delayed in Nod2-/- mice. Neonatal antibiotic treatment significantly changed the microbiota at weaning in WT and Nod2-/- littermates; however, Nod2-/- mice maintained reduced microbial diversity 14 days after cessation of antibiotics. Although treatment of adult mice did not influence susceptibility to colitis, neonatally treated Nod2-/- mice developed a more severe colitis. Moreover, the colitis phenotype was transferable through fecal transplantation into germ-free Nod2-/- recipients, and was associated with changes in intestinal T cells and the cytokine milieu following inflammation. These data demonstrate that neonatal antibiotic exposure has long-lasting influence on the microbiota and mucosal immunity, and may explain how NOD2 contributes to the risk of intestinal inflammation. |
| Databáze: | OpenAIRE |
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