MOLECULAR EPIDEMIOLOGY OFCANINE PARVOVIRUS TYPE2A AND 2B AMONG SHELTER DOGS — THE NEW RECOMBINATION IN VIRUS
| Autor: | Man-Yu Hsu, Kuan-Hua Chen, Yu Ching Lan, Michelle Liang, Yi-Chen Yang, Ming-Hua Sung, Yuan-Ting Lu, Day-Yu Chao, Tsung-Ming Chen |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genetics Genetic diversity biology Molecular epidemiology Phylogenetic tree animal diseases viruses Canine parvovirus biology.organism_classification Virology Virus law.invention 03 medical and health sciences 030104 developmental biology law Viral evolution Polymerase chain reaction Recombination |
| Zdroj: | Taiwan Veterinary Journal. 43:47-53 |
| ISSN: | 2382-5588 1682-6485 |
| DOI: | 10.1142/s1682648515500341 |
| Popis: | Canine parvovirus type 2 (CPV-2) is a severe enteric pathogen mainly affecting dogs. CPV-2 contains three antigenic variants (2a, 2b, and 2c) that are distributed internationally. Detection and characterization of the currently circulating CPV-2 strains are vital for the understanding of viral evolution, transmission, and the development of methods to control its spreading. Herein, we analyzed the strains in central Taiwan to provide information of local viral evolution, diversity, and epidemiology. Stool and blood samples from 24 dogs and 2 cats were genotyped by PCR amplification of strain-specific VP2 sequence collected during 2011–2013. 60% (16/26) of them were positive and 100% (16/16) of these positive samples were type 2b. Then, the full length VP2 gene was sequenced in 6 CPV-positive samples and a maximum-likelihood phylogenetic tree was constructed using both Taiwan and other strains worldwide. Surprisingly, all Taiwan CPVs showed high relatedness to type 2a. Recombination analysis revealed a recombination of VP2 gene between type 2a and 2b. This study demonstrates a recombination between CPV-2a and 2b in nature that contributes to the genetic diversity and evolution of CPV-2. |
| Databáze: | OpenAIRE |
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