Transient and stable expression of hepatitis B surface antigen in tomato (Lycopersicon esculentum L.)

Autor:	Vishwas A. Bapat, Thumballi R. Ganapathi, G. B. Sunil Kumar, C. J. Revathi, L. Srinivas
Rok vydání:	2008
Předmět:	Hepatitis B virus Agroinfiltration biology fungi food and beverages Plant Science medicine.disease_cause biology.organism_classification Hepatitis b surface antigen Virology Molecular biology Lycopersicon Molecular analysis Antigen medicine Genetically modified tomato Gene Biotechnology
Zdroj:	Plant Biotechnology Reports. 2:1-6
ISSN:	1863-5474 1863-5466
DOI:	10.1007/s11816-008-0041-z
Popis:	Cotyledonary leaves of tomato cv. Megha were transformed with the hepatitis B virus ‘s’ gene, which encodes surface antigen. Six plant expression cassettes (pHBS, pHER, pEFEHBS, pEFEHER, pSHER and pEFESHER) were used to assay the possible expression levels by agroinfiltration. The maximum transient expression level of 489.5 ng/g D.W. was noted in pEFEHER-infiltrated cotyledonary leaves. Transgenic tomato plants with pEFEHBS and pEFEHER expression cassettes were regenerated and characterized by molecular analysis. The expression of the antigen in the fruits was confirmed by RT-PCR and ELISA analysis. This is the first report on the expression of hepatitis B surface antigen in tomato.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::bab23db43fb307c5be3031f3415096b5 https://doi.org/10.1007/s11816-008-0041-z Zobrazit plný text záznamu Full text from SpringerLink