Algae support populations of heterotrophic, nitrifying, and phosphate-accumulating bacteria in the treatment of poultry litter anaerobic digestate
| Autor: | Elizabeth M. Bankston, Qichen Wang, Brendan T. Higgins |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Chlorella sorokiniana
biology Chemistry General Chemical Engineering Microorganism Candidatus Accumulibacter 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology biology.organism_classification 01 natural sciences Industrial and Manufacturing Engineering 0104 chemical sciences Algae Nitrifying bacteria Digestate Environmental Chemistry Green algae Food science 0210 nano-technology Nitrospira |
| Zdroj: | Chemical Engineering Journal. 398:125550 |
| ISSN: | 1385-8947 |
| Popis: | The objective of this study was to investigate the impact that algae have on bacterial treatment of poultry litter slurry anaerobic digestate. We hypothesized that algae could provide benefits to heterotrophic and nitrifying organisms, due in part to provision of dissolved oxygen. No other studies, to our knowledge, have shown that algae provide benefits to nitrifying bacteria in the treatment of anaerobic digestates. Batch cultures of the native digestate community were grown alone or with the addition of activated sludge microorganisms and/or the green algae, Chlorella sorokiniana. Water quality measurements, 16S rRNA sequencing, and quantitative PCR were utilized to elucidate the impacts of algae on bacterial populations involved in organics removal and nutrient cycling. The presence of activated sludge promoted algal growth by 29%, nitrogen uptake by 53%, and phosphate removal by 21%. In turn, the presence of algae promoted a 57% increase in organics removal by heterotrophic bacteria. Algae also promoted full oxidation of ammonium to nitrate by nitrifying bacteria whereas incomplete nitrification was observed in the absence of algae. This result was likely driven by algal support of the commamox bacteria, Nitrospira, which otherwise died off in the anaerobic digestate. We also show here, for the first time, that C. sorokiniana sustained the relative abundance of the phosphate-accumulating bacteria, Candidatus Accumulibacter, whereas this organism declined in relative abundance by 94% when algae were absent (p |
| Databáze: | OpenAIRE |
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