Serendipitous discovery of a novel protostrongylid (Nematoda: Metastrongyloidea) in caribou, muskoxen, and moose from high latitudes of North America based on DNA sequence comparisons

Autor: Dorothy Cooley, Eric P. Hoberg, Ingrid AsmundssonI. Asmundsson, Greg D. Appleyard, Alasdair VeitchA. Veitch, Neil B. Chilton, Richard Popko, Marsha Branigan, Florence Huby-Chilton, Emily J. Jenkins, Michelle OakleyM. Oakley, Lydden Polley, Kimberlee BeckmenK. Beckmen, Lem ButlerL. Butler, B. Elkin, Manon Simard, Aedes ScheerA. Scheer, Deborah JohnsonD. Johnson, Susan J. Kutz, John NagyJ. Nagy, Abdurakhim KuchboevA. Kuchboev
Rok vydání: 2007
Předmět:
Zdroj: Canadian Journal of Zoology. 85:1143-1156
ISSN: 1480-3283
0008-4301
Popis: Fecal samples are often the only feasible means to assess diversity of parasites in wildlife; however, definitive identification of egg or larval stages in feces by morphology is rarely possible. We determined partial sequences from the second internal transcribed spacer region (ITS-2) of nuclear ribosomal DNA for first-stage, dorsal-spined larvae (DSL) in feces from caribou ( Rangifer tarandus tarandus (L., 1758), Rangifer tarandus caribou (Gmelin, 1788), Rangifer tarandus grantii (Allen, 1902)), muskoxen ( Ovibos moschatus moschatus (Zimmermann, 1780), Ovibos moschatus wardi Lydekker, 1900), moose ( Alces alces gigas Miller, 1899 and Alces alces andersoni Peterson, 1952), and from the tissue of one slug ( Deroceras laeve (Müller, 1774)) in Arctic–Subarctic North America. A previously uncharacterized, genetically distinct species was recognized based on sequences of 37 DSL from 19 ungulate hosts and the slug. Sequence similarity among individuals of this novel species was 91%–100%. For many individual DSL, paralogues of ITS-2 were detected. ITS-2 sequences from the novel species were 72%–77% similar to those of Varestrongylus alpenae (Dikmans, 1935) and 51%–61% similar to those of other protostrongylids known in North American and some Eurasian ungulates. Results indicate a discrete lineage of an undescribed protostrongylid infecting muskoxen, caribou, and moose from Alaska to Labrador. Sympatric infections with Parelaphostrongylus andersoni Prestwood, 1972 were found in three caribou herds.
Databáze: OpenAIRE
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