Non-invasive high throughput approach for protein hydrophobicity determination based on surface tension

Autor:	Katharina Christin Bauer, Lara Galm, Sven Amrhein, Jürgen Hubbuch
Rok vydání:	2015
Předmět:	Work (thermodynamics) Chromatography Bioengineering Bromophenol blue Applied Microbiology and Biotechnology Surface tension Stalagmometric method Hydrophobic effect chemistry.chemical_compound chemistry Lysozyme Hydrophobicity scales Biotechnology Egg white
Zdroj:	Biotechnology and Bioengineering. 112:2485-2494
ISSN:	0006-3592
DOI:	10.1002/bit.25677
Popis:	The surface hydrophobicity of a protein is an important factor for its interactions in solution and thus the outcome of its production process. Yet most of the methods are not able to evaluate the influence of these hydrophobic interactions under natural conditions. In the present work we have established a high resolution stalagmometric method for surface tension determination on a liquid handling station, which can cope with accuracy as well as high throughput requirements. Surface tensions could be derived with a low sample consumption (800 μL) and a high reproducibility (
Databáze:	OpenAIRE
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