Reverse Phase HPLC For Rapid, Comprehensive Measurement of Nucleotides, Nucleosides and Bases of The Myocardial Adenine Pool
| Autor: | S.M. Humphrey, D. G. Holliss, R.N. Seelye, M. A. Morrison |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
chemistry.chemical_classification
Inosine monophosphate Chromatography Biochemistry (medical) Clinical Biochemistry Nicotinamide adenine dinucleotide Biochemistry Adenosine Analytical Chemistry chemistry.chemical_compound chemistry Adenine nucleotide Electrochemistry medicine Nucleotide NAD+ kinase Inosine Spectroscopy Hypoxanthine medicine.drug |
| Zdroj: | Analytical Letters. 17:2047-2065 |
| ISSN: | 1532-236X 0003-2719 |
| DOI: | 10.1080/00032718408077197 |
| Popis: | A novel reverse phase HPLC method is described for the simultaneous measurement of adenosine tri-, di- and monophosphates (ATP, ADP, AMP), inosine monophosphate (IMP), adenosine, inosine, hypoxanthine, nicotinamide adenine dinucleotide (NAD) and uric acid in cardiac tissues and coronary effluent. The use of a simplified perchloric acid extraction procedure and ODS columns easily modified with Mq++, Tris and phosphate buffer, allows considerable saving in analysis time together with extremely good resolution, particularly for ATP and ADP, and provides a very practical tool for the routine assessment of changes in adenine pool metabolites. |
| Databáze: | OpenAIRE |
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