Fluid Reabsorption by the Ductuli Efferentes Testis of the Rat Is Dependent on Both Sodium and Chlorine1
| Autor: | Russell C. Jones, L. A. Hansen, S. Y. Man, John Clulow, Françoise Dacheux |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Reabsorption Sodium chemistry.chemical_element Efferent ducts Cell Biology General Medicine Biology Inorganic ions Epididymis Epithelium chemistry.chemical_compound medicine.anatomical_structure Endocrinology Reproductive Medicine chemistry Internal medicine medicine Choline Mannitol medicine.drug |
| Zdroj: | Biology of Reproduction. 71:410-416 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod.104.027490 |
| Popis: | The role of Na1 and Cl2 in fluid reabsorption by the efferent ducts was examined by perfusing individual ducts in vivo with preparations of 160 mM NaCl in which the ions were replaced, together or individually, with organic solutes while maintaining the osmolality at 300 mmol/kg. Progressively replacing NaCl with mannitol reduced net reabsorption of water and the ions in a concentration-dependent manner, and caused net movement into the lumen at concentrations of NaCl less than 80 mM. The net rates of flux were lower for Na1 than for Cl2. In collectates, [Na1] was greater than [Cl2], indicating that Cl2 transport is probably linked with another anion. Replacing either Na1 or Cl2 in perfusates (with choline and isethionate, respectively) while maintaining the other inorganic ion at 160 mM also reduced net rates of reabsorption in a concentration-dependent manner to zero when either ion was completely replaced. There were no significant differences in the osmolality of perfusate and collectate, and collectates contained a mean of 3.4 mM K1, indicating a backflux of K1 into the lumen. It is concluded that fluid reabsorption from the efferent ducts is dependent on the transport of both Na1 and Cl2 from the lumen (from a luminal concentration of at least 70‐80 mM), and that Cl2 transport is dependent on another anion. The epithelium is permeable to K1 and has a higher permeability to a range of organic solutes (mannitol, choline, and isethionate) than epithelium in the proximal kidney tubules. epididymis, male reproductive tract |
| Databáze: | OpenAIRE |
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