A Novel In Vitro Assay Correlates Insulin Receptor Autoantibodies With Fasting Insulin in Type B Insulin Resistance
| Autor: | Waldemar B Minich, Brent S Abel, Christian Schwiebert, Tim Welsink, Petra Seemann, Rebecca J Brown, Lutz Schomburg |
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| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/clinem/dgad125 |
| Popis: | ContextSevere insulin resistance (IR) in the presence of insulin receptor autoantibodies (InsR-aAb) is known as type B insulin resistance (TBIR). Considerable progress in therapy has been achieved, but diagnosis and monitoring of InsR-aAb remains a challenge.ObjectiveThis work aimed to establish a robust in vitro method for InsR-Ab quantification.MethodsLongitudinal serum samples from patients with TBIR at the National Institutes of Health were collected. A bridge-assay for InsR-aAb detection was established using recombinant human insulin receptor as bait and detector. Monoclonal antibodies served as positive controls for validation.ResultsThe novel assay proved sensitive, robust, and passed quality control. The measured InsR-aAb from TBIR patients was associated with disease severity, decreased on treatment, and inhibited insulin signaling in vitro. Titers of InsR-aAb correlated positively to fasting insulin in patients.ConclusionQuantification of InsR-aAb from serum samples via the novel in vitro assay enables identification of TBIR and monitoring of successful therapy. |
| Databáze: | OpenAIRE |
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