Changes in expression of NLRP3 inflammasome components and oxidative parameters of mice subjected to high-fat diet and rosa mosqueta oil supplementation
| Autor: | Daniel González-Mañán, Gladys Tapia, Camila G. Dossi |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
medicine.medical_specialty
Antioxidant Insulin medicine.medical_treatment Adipose tissue Inflammasome Biology Carbohydrate medicine.disease medicine.disease_cause Biochemistry Endocrinology Insulin resistance Physiology (medical) Internal medicine medicine Metabolic syndrome Oxidative stress medicine.drug |
| Zdroj: | Free Radical Biology and Medicine. 108:S23 |
| ISSN: | 0891-5849 |
| DOI: | 10.1016/j.freeradbiomed.2017.04.102 |
| Popis: | Introduction Rosa mosqueta oil (RM) is high in alpha-linolenic acid (ALA) and tocopherols (α-, γ-), which have anti-inflammatory, antioxidant and insulin sensitizing properties, thus preventing the high-fat diet (HFD)-induced damage in mice. Objective To evaluate changes in NLRP3 inflammasome components expression and oxidative parameters in HFD-fed mice supplemented with RM. Methods C57BJ/6 J mice (n=9/group) were fed for 12 weeks and divided into: (i) control diet (CD, 20% proteins, 70% carbohydrates, 10% lipids); (ii) CD+RM (0.01 mL/g bodyweight/day); (iii) HFD (20% protein, 20% carbohydrate, 60% lipids); (iv) HFD+RM. Oxidative stress (carbonylated proteins, MDA content and Nrf2 levels) and NLRP3 inflammasome components (NLRP3, ASC, Caspase-1, IL-1beta) expression in liver and visceral adipose tissue were determined. Results: HFD+RM group showed significantly decreased (two-way ANOVA, bonferroni test, P Conclusion Dietary RM supplementation decreases NLRP3 inflammasome expression and oxidative stress. This data could be associated with the prevention of metabolic syndrome. FONDECYT 1140547 |
| Databáze: | OpenAIRE |
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