| Autor: |
A.S. Speranskaya, I.V. Artyushin, A.E. Samoilov, E.V. Korneenko, K. Khabudaev, E.N. Ilina, A.P. Yusefovich, M.V. Safonova, A.S. Dolgova, A.S. Gladkikh, V.G. Dedkov, Daszak Peter |
| Rok vydání: |
2022 |
| DOI: |
10.1101/2022.06.09.495421 |
| Popis: |
The majority of emerging infectious diseases are caused by pathogens with zoonotic origin, and most of these emerged from wildlife reservoirs. Bats are diverse, and widely distributed globally, and are the known or hypothesized reservoir of a series of emerging zoonotic viruses. Analyses of bat viromes have been used to identify novel viruses with potential to cause human infection. We characterized the fecal virome of 26 samples collected from six bat species captured during 2015 in Moscow Region. Of these 13/26 (50%) samples were found to be coronavirus positive. We sequenced and assembled the complete genome of a novel MERS-related Betacoronavirus fromPipistrellus nathusii, named MOW-BatCoV strain 15-22. OfP. nathusii3/6 samples were found to carriers of MOW-BatCoVs. The genome organization of MOW-BatCoV/15-22 was identical to other known MERS-related coronaviruses. Phylogenetic analysis of whole genomes suggests that MOW-BatCoV/15-22 falls into a distinct subclade closely related to human and camel MERS-CoV, and MERS-related CoVs from the bat speciesHypsugo saviiandPipistrellus kuhlii(from Italy) andNeoromicia capensis(from South Africa). Unexpectedly, phylogenetic analysis of the novel MOW-BatCoV 15-22 spike genes showed the closest similarity to a bat CoV Neoromicia/5038 and CoVs fromErinaceus europaeus(the European hedgehog), thus MOW-BatCoV could arise as result of recombination between ancestral viruses of bats and hedgehogs. Computer molecular docking analysis of MOW-BatCoV 15-22 Spike glycoprotein binding to DPP4 receptors of different mammal species predicted highest binding interaction with DPP4 of the batM. brandtii(docking score -320.15) and the European hedgehog,E. europaeus(docking score -294.51). Hedgehogs are widely kept as pets, and are commonly found in areas of human habitation. Our finding of a novel bat-CoV likely able to infect hedgehogs suggests the potential for hedgehogs to act as intermediate hosts for bat-CoVs between bats and humans. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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