Bioanalytical Application of SERS Immunoassay for Detection of Prostate-Specific Antigen

Autor:	Dongjin Pyo, Kyung Jin Yoon, Jong Hoon Hahn, Young Mee Jung, In-Yong Eom, Hyeong Kuyn Seo, Hoon Hwang
Rok vydání:	2010
Předmět:	Bioanalysis Chromatography medicine.diagnostic_test Chemistry Substrate (chemistry) Nanotechnology General Chemistry symbols.namesake Antigen Colloidal gold Immunoassay Monolayer medicine symbols Raman spectroscopy Raman scattering
Zdroj:	Bulletin of the Korean Chemical Society. 31:1215-1218
ISSN:	0253-2964
DOI:	10.5012/bkcs.2010.31.5.1215
Popis:	We demonstrate the possible application of the sandwich type surface-enhanced Raman scattering (SERS) immuno-assay using antigen-antibody binding for detection of prostate-specific antigen (PSA) in cancer cells. In this sandwich type of SERS immunoassay, to capture antigens onto the immobilized layer of antibodies on the gold substrate we prepared the monolayer of gold nanoparticles on the APTMS-derivatized surface of a glass slide by using the SAM technique. This sandwich type of SERS immunoassay in which antigens on the substrate specifically capture antibodies on a Raman reporter (DSNB coated gold nanoparticles with R6G) could successfully detect PSA at low levels. A strong SERS spectrum of Raman reporter was observed only with a substrate in which PSA is present.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ba5e92d2eb160eabab77f1bbc85bcb3d https://doi.org/10.5012/bkcs.2010.31.5.1215 Zobrazit plný text záznamu