Biogenic selenium nanoparticles produced by Lactobacillus casei ATCC 393 inhibit colon cancer cell growth in vitro and in vivo
| Autor: | C. Sarafidis, Eleni Tryfonopoulou, Georgios Aindelis, Katerina Chlichlia, Petros Ypsilantis, Orestis Kalogirou, Katerina Spyridopoulou |
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| Rok vydání: | 2021 |
| Předmět: |
inorganic chemicals
Lactobacillus casei chemistry.chemical_element Bioengineering 02 engineering and technology law.invention 03 medical and health sciences Probiotic In vivo law General Materials Science 030304 developmental biology chemistry.chemical_classification 0303 health sciences Reactive oxygen species biology Chemistry General Engineering food and beverages General Chemistry 021001 nanoscience & nanotechnology biology.organism_classification Atomic and Molecular Physics and Optics In vitro Bioavailability Biochemistry Cancer cell 0210 nano-technology Selenium |
| Zdroj: | Nanoscale Advances. 3:2516-2528 |
| ISSN: | 2516-0230 |
| Popis: | Selenium compounds exhibit excellent anticancer properties but have a narrow therapeutic window. Selenium nanoparticles, however, are less toxic compared to other selenium forms, and their biogenic production leads to improved bioavailability. Herein, we used the probiotic strain Lactobacillus casei ATCC 393, previously shown to inhibit colon cancer cell growth, to synthesize biogenic selenium nanoparticles. We examined the anticancer activity of orally administered L. casei, L. casei-derived selenium nanoparticles and selenium nanoparticle-enriched L. casei, and investigated their antitumor potential in the CT26 syngeneic colorectal cancer model in BALB/c mice. Our results indicate that L. casei-derived selenium nanoparticles and selenium nanoparticle-enriched L. casei exert cancer-specific antiproliferative activity in vitro. Moreover, the nanoparticles were found to induce apoptosis and elevate reactive oxygen species levels in cancer cells. It is noteworthy that, when administered orally, selenium nanoparticle-enriched L. casei attenuated the growth of colon carcinoma in mice more effectively than the isolated nanoparticles or L. casei, suggesting a potential additive effect of the nanoparticles and the probiotic. To the best of our knowledge this is the first comparative study examining the anticancer effects of selenium nanoparticles synthesized by a microorganism, the selenium nanoparticle-enriched microorganism and the sole microorganism. |
| Databáze: | OpenAIRE |
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