Over-expression of highly conserved mitochondrial 70-kDa heat-shock protein in the sea anemone Anemonia viridis

Autor:	Omer Choresh, Abdussalam Azem, Yossi Loya
Rok vydání:	2007
Předmět:	chemistry.chemical_classification biology Physiology Marine invertebrates Mitochondrion Sea anemone biology.organism_classification Biochemistry Anemonia Amino acid Hsp70 chemistry Affinity chromatography Heat shock protein Botany General Agricultural and Biological Sciences Developmental Biology
Zdroj:	Journal of Thermal Biology. 32:367-373
ISSN:	0306-4565
DOI:	10.1016/j.jtherbio.2007.04.006
Popis:	Organisms react to cellular stress by inducing the synthesis of heat-shock proteins (Hsp). One such protein is the mitochondrial 70-kDa Hsp (mHsp70). The expression of mHsp70 in organisms that undergo stress in their natural habitat is unknown. We used a biochemical approach enabling to identify an mHsp70 from the sea anemone Anemonia viridis , which is abundant in highly fluctuating marine habitats. Antibodies raised specifically against yeast mHsp70 recognized a 70-kDa protein from A. viridis . We found that A. viridis mHsp70 is constitutively expressed at 22–23 °C, but over-expressed upon exposure to heat shock (31 °C) or to temperature fluctuations, suggesting that mHsp70 plays a significant role in adaptation of sessile marine invertebrates to highly fluctuating environmental conditions. Using an affinity column we were able to obtain a partially purified fraction of this protein. Partial amino acid sequences proved that the purified Hsp70 functions in the mitochondria.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ba43f3083c05134b2973919df239173a https://doi.org/10.1016/j.jtherbio.2007.04.006 Zobrazit plný text záznamu Full Text from ScienceDirect