Effects of Microgravity on the Virulence ofSalmonellaTowardCaenorhabditis elegans
| Autor: | Timothy G. Hammond, Jeanne L Becker, Holly H. Birdsall, Jeffrey S. Hammond, Paul Koenig, Louis S. Stodieck, Margaret A. Gunter, Patricia L. Allen |
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| Rok vydání: | 2013 |
| Předmět: |
Salmonella
Microorganism Energy Engineering and Power Technology Aerospace Engineering Virulence Astronomy and Astrophysics Biology Spaceflight biology.organism_classification medicine.disease_cause Pathogenicity island Microbiology law.invention Simulated microgravity law Tourism Leisure and Hospitality Management medicine Safety Risk Reliability and Quality Gene Caenorhabditis elegans |
| Zdroj: | New Space. 1:123-131 |
| ISSN: | 2168-0264 2168-0256 |
| DOI: | 10.1089/space.2013.0011 |
| Popis: | To evaluate the effects of microgravity on virulence genes in Salmonella, we studied the ability of various Salmonella deletion mutants to kill wild-type Caenorhabditis elegans nematodes at the larval and adult stages. Simultaneous studies were performed utilizing spaceflight, clinorotation, and static ground controls. Nematodes, Salmonella, and growth media were separated until exposed to true or simulated microgravity, and then mixed and grown for 48 h. Experiments were terminated by paraformaldehyde fixation, and optical density measurements were used to assay residual microorganisms. Prior flight in space led to reduced virulence of wild-type Salmonella when subsequently evaluated in a ground-based virulence assay with carefully matched inocula for never-flown Salmonella controls. However, when the virulence assay was conducted in spaceflight, there was only a minimal change in the virulence of wild-type Salmonella toward C. elegans. Deletion of pipA, a gene in Salmonella pathogenicity island... |
| Databáze: | OpenAIRE |
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