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Summary Cellulose-producing bacteria used for Nata de Coco production in the Philippines were isolated and characterized. Two distinct wild-type strains (ITDI 2.1 and PA 2.2) were selected from thirty-eight isolates obtained. The two strains, and the rest of the cellulose producers, were established to be members of the genus Acetobacter based on rapid phenotypic characterization. They were characterized and differentiated based on pellicle type and colony morphology, carbon-utilization pattern (Biolog™ assay), amount of cellulose production and 16S rDNA sequence. The thick pellicle cellulose-producing strain (ITDI 2.1) has a stable and consistent production of a very thick pellicle in any sugar-based, acidic medium, with a dry weight up to twenty-six times that of the thin pellicle cellulose-producing strain (PA 2.2) after 7–8 days static batch culture. Species identification using 16S rDNA sequencing revealed that the two strains belong to two different species of Acetobacter, Acetobacter xylinus and A. hansenii and may be new subspecies under these species designation. |
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