Synthesis of 7-amino-4-methylcoumarin (AMC) derivatives and their hydrolysis by plant cysteine proteinases
| Autor: | J. M. Sullivan, C. J. Gray |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
musculoskeletal diseases
chemistry.chemical_classification Bromelain (pharmacology) Renewable Energy Sustainability and the Environment General Chemical Engineering Organic Chemistry Peptide Pollution Inorganic Chemistry Serine chemistry.chemical_compound Papain Hydrolysis Fuel Technology Enzyme chemistry Biochemistry Peptide synthesis Waste Management and Disposal Biotechnology Cysteine |
| Zdroj: | Journal of Chemical Technology & Biotechnology. 46:11-26 |
| ISSN: | 1097-4660 0268-2575 |
| Popis: | A number of derivatives of citrullyl-7-amino-4-methylcoumarin (Cit-AMC) have been synthesised and characterised. Their hydrolysis, releasing the fluorescent 7-amino-4-methylcoumarin, by the plant cysteine proteinases papain, ficin and bromelain was examined. Two derivatives in particular, Z-Phe-Cit-AMC and Z-Gly-Phe-Cit-AMC, were shown to be good substrates for these enzymes. The possible use of the fluorogenic peptides in assays for these enzymes is discussed. |
| Databáze: | OpenAIRE |
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