Mutation to Increase Sporulation of a Strain ofHirsutella citriformisfrom Mexico and Evaluation againstDiaphorina citri
| Autor: | Georgina Cruz-Juárez, Raúl Rodríguez-Guerra, Hamlet Avilés-Arnaut, María del Socorro Flores-González, María Guadalupe Maldonado-Blanco, Susana De la Torre-Zavala |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Diaphorina Oryza sativa Ecology biology Candidatus Liberibacter Diaphorina citri Hypocreales Hirsutella food and beverages Methane sulfonate biology.organism_classification 01 natural sciences Conidium 010602 entomology 03 medical and health sciences Horticulture 030104 developmental biology Insect Science Agronomy and Crop Science |
| Zdroj: | Southwestern Entomologist. 43:891-904 |
| ISSN: | 2162-2647 0147-1724 |
| Popis: | Huanglongbing is an important citrus disease caused by the bacterium Candidatus liberibacter and transmitted by the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liividae). Psyllids are suppressed by chemical and biological controls including several entomopathogenic fungi such as Hirsutella citriformis Speare (Hypocreales: Ophiocordycipitaceae). A native strain of H. citriformis INIFAP-Hir 2 from Mexico was cultured in two substrates, rice (Oryza sativa L.) and oat (Avena sativa L.) in two types of containers (plastic and glass). The fungal strain was exposed to different concentrations of ethyl methane sulfonate to obtain mutants with improved production and germination of conidia, while maintaining virulence against the Asian citrus psyllid. Mutants were selected based on the amount of synnemata and sporulation. Four putative mutants were obtained, with 53-10 producing the most conidia in potato dextrose with 1% yeast extract, and also on oatmeal, compared with the wild strain. The mutant and wild strains killed 100 and 94.4%, respectively, of Asian citrus psyllids. Mycosis on adults was 60.5 and 73.4% by wild and mutant strains, respectively. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|