Two-dimensional electrophoretic analysis of cytosols from human breast tumors: optimal migration conditions

Autor:	J.C. Delarue, Bohuon C, Comoy E, Jean-Michel Bidart, P Motté, Ph. Moingeon
Rok vydání:	1984
Předmět:	chemistry.chemical_compound Electrophoresis Chromatography chemistry Homogeneous Acrylamide Biochemistry (medical) Clinical Biochemistry Ph gradient Sodium dodecyl sulfate Human breast
Zdroj:	Clinical Chemistry. 30:1947-1949
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/30.12.1947
Popis:	In an examination of cytosols from human breast tumors, we performed two-dimensional electrophoretic analysis. Several migration conditions were tried in a search for a homogeneous repartition of cytosolic proteins. The most workable gels were obtained with a 4.40 to 8.05 pH gradient in the first dimension and a homogeneous 125 g/L acrylamide/sodium dodecyl sulfate gel, 1 mm in thickness, in the second dimension. The practicability of this method is discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ba243a09487db9c9fb3130ed5dd09d5a https://doi.org/10.1093/clinchem/30.12.1947 Zobrazit plný text záznamu