Evaluation of STR profiles of single telogen hairs using probabilistic methods

Autor:	Melanie Grabmüller, Ralf Thiele, Sarah Aurora Heß, Burkhard Madea, Jan-Philip Biermann, Richard Jäger
Rok vydání:	2019
Předmět:	Telogen hair chemistry.chemical_compound Capillary electrophoresis integumentary system STR analysis chemistry Genetics Degraded dna Computational biology Biology DNA sequencing DNA Pathology and Forensic Medicine
Zdroj:	Forensic Science International: Genetics Supplement Series. 7:454-456
ISSN:	1875-1768
DOI:	10.1016/j.fsigss.2019.10.049
Popis:	Full STR profiles can rarely be obtained from single telogen hairs due to the minute amount and compromised integrity of their DNA. We developed a next generation sequencing (NGS)-based STR assay, called maSTR (mini-amplicon STR) assay that is particularly suited for degraded DNA. The maSTR assay yielded higher allele recoveries than capillary electrophoresis (CE)-based STR analysis from DNA from single telogen hairs, however displayed a higher number of allelic drop-ins. To calculate likelihood ratios for assignment to correct donors, profiles composed by consensus or composite interpretation were subjected to fully or semicontinuous probabilistic methods using the GenoProof Mixture 3 software. It was possible to assign profiles from single telogen hairs to their correct donor profiles if DNA was of sufficient quality.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ba088402c612d3a64f2cede444e14460 https://doi.org/10.1016/j.fsigss.2019.10.049 Zobrazit plný text záznamu Full Text from ScienceDirect