Lack of Specificity of Cyclosporine Immunoassays

Autor:	David L. Witte, Edward Wang, Steven J. Soldin, Ronald J. Elin, Bernard W. Steele
Rok vydání:	2003
Předmět:	Chromatography medicine.diagnostic_test biology Chemistry Metabolite General Medicine Mass spectrometric Pathology and Forensic Medicine Medical Laboratory Technology chemistry.chemical_compound Polyclonal antibodies Immunoassay medicine biology.protein Proficiency testing
Zdroj:	Archives of Pathology & Laboratory Medicine. 127:19-22
ISSN:	1543-2165 0003-9985
Popis:	Objective.—To evaluate the cross-reactivity of the 6 most abundant cyclosporine A (CsA) metabolites in commonly used assays for CsA. Design.—Whole blood samples containing either only 62 ng/mL CsA (A) or 62 ng/mL CsA and between 49 and 86 ng/mL of 1 of the 6 most abundant CsA metabolites (B) were lyophilized. One sample of A and 1 of B were mailed to each of the laboratories participating in the College of American Pathologists Proficiency Testing Program quarterly during a 3-year period (1999–2001). Method means and coefficients of variation were calculated for each mailing. Results.—The study showed significant cross-reactivity of metabolites in all the immunoassay systems studied. Overall degree of interference decreased from Abbott TDx polyclonal > Abbott TDx monoclonal > DiaSorin > Syva EMIT. High-performance liquid chromatography methods gave results close to those found using mass spectrometric techniques. Conclusions.—Significant metabolite interference was found to occur with the immunoassay systems studied.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ba063fcf566759a208abc08c83a50fe4 https://doi.org/10.5858/2003-127-19-losoc Zobrazit plný text záznamu Plný text ve formátu PDF