Protein synthesis in mammalian cells with particular reference to antibody formation
| Autor: | Brigitte A. Askonas |
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| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Recueil des Travaux Chimiques des Pays-Bas. 77:611-622 |
| ISSN: | 0165-0513 |
| DOI: | 10.1002/recl.19580770703 |
| Popis: | Recent work concerned with protein biosynthesis in mammalian systems is reviewed. Antibody protein was chosen as an example of a specific protein and its formation and secretion was studied in slices of lymphoid and reticuloendothelial organs of immunized animals. [1 C] Amino acids added to the incubation medium appeared in intracellular antibody extractable with saline after a lag period of 10–15 min, suggesting that antibody is first formed bound to subcellular particles. There is a longer lag period (more than 30 min) before the newly formed radioactive antibody is secreted by the cell into the extracellular medium. Plasma cells which are responsible for antibody formation have a highly organized endoplasmic reticulum similar to hepatic and pancreatic cells known to actively secrete proteins. Microsomal fraction contains bound antibody which may be released by destroying the structure of the particles and it probably represents the site of antibody formation. So far antibody synthesis by microsomes could not be demonstrated since mild damage or breakage of the cells stopped incorporation of radioactive amino acids into antibody extractable with saline. |
| Databáze: | OpenAIRE |
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