Isolation and characterization of thePhysarum polycephalumextracellular polysaccharides
Autor: | George T. Sperl |
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Rok vydání: | 1990 |
Předmět: |
chemistry.chemical_classification
Chromatography biology Magnesium chemistry.chemical_element Physarum polycephalum Fraction (chemistry) Calcium biology.organism_classification Polysaccharide Applied Microbiology and Biotechnology chemistry.chemical_compound chemistry Biochemistry Acetone Fermentation Sugar Food Science Biotechnology |
Zdroj: | Food Biotechnology. 4:663-668 |
ISSN: | 1532-4249 0890-5436 |
DOI: | 10.1080/08905439009549781 |
Popis: | The extracellular polysaccharide formed by Physarum polycephalum during spherulation was produced by fermentation. Both cell yields and polysaccharide formation were adversely affected by the presence of ambient incident light. Crude polysaccharide was fractionated with acetone into two fractions (I, II). Fraction I was viscous and contained 3.5% sulfated and 0.5% phosohorylated sugar residues. The non‐viscous Fraction II contained 0.4% sulfated and 22% phosphorylated sugar residues. Fraction II formed gels at pH>8.5 with calcium, magnesium, iron and zinc ions. Fraction I and unfractioned polysaccharide did not form gels under any conditions tested. |
Databáze: | OpenAIRE |
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