| Autor: |
Vlad David, Maria Stanciulescu, Florin Horhat, Sharma Abhinav, Nilima Kundnani, Bogdan Ciornei, Marius Popoiu, Eugen Boia |
| Rok vydání: |
2020 |
| DOI: |
10.21203/rs.3.rs-136386/v1 |
| Popis: |
Introduction: Many consider overgrowth of the costal cartilages to be the etiological factor for chest wall deformities in children. The aim of this study is to investigate if induced overgrowth of the costal cartilages leads to the deformation of the chest wall in an animal model. Material and Methods: We injected insulin growth factor 1 (IGF1) solution directly under the perichondrium of the last three costal cartilages of rat pups. We used two different concentrations: 50 μg/ml (E50) and 100 μg/ml (E100). The procedure was repeated once per week for five consecutive weeks. Seven days from the last administration of injections, the animals were euthanized. We assessed the shape and measured the diameters of the thoracic cage. The last three costal cartilages were dissected; the samples were prepared and examined in light microscopy. Results: In E100 the sagittal and coronal diameters of the rib cage were larger than E50 and control groups but without any deformation of the chest wall. The microscopic examinations revealed an anabolic pattern in E100.Conclusion: Locally administered IGF1 stimulates, in a dose dependent manner, in-vivo cellular growth and multiplication in the costal cartilages. The induced overgrowth of the costal cartilages however, didn’t result in the deformation of the chest wall. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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