Study of RNA polymerase transcription through nucleosome using the cryo-electron microscopy approach
| Autor: | Vasily M. Studitsky, Olga Sokolova, M. G. Karlova, Fu Kai Hsieh, O. I. Volokh, E. S. Trifonova |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cryo-electron microscopy Biology Molecular biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Transmission microscopy Transcription (biology) RNA polymerase Microscopy Biophysics Nucleosome General Agricultural and Biological Sciences General Environmental Science Macromolecule |
| Zdroj: | Moscow University Biological Sciences Bulletin. 71:34-38 |
| ISSN: | 1934-791X 0096-3925 |
| Popis: | A promising and reliable method for the analysis of macromolecule structures is capturing images using cryo-electron transmission microscopy and consequently performing three-dimensional reconstructions. In this study, using cryo-electron microscopy, we analyzed the structure of the complex, formed by RNA polymerase stalled at position +42 during its transcription through the nucleosome. We obtained both projection images and a three-dimensional structure of the EC-42 complex at 2.5 nm resolution. This allowed us to confirm the conformational integrity of the nucleosome during the passage of the RNA polymerase. |
| Databáze: | OpenAIRE |
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