Regeneration in sponge Sycon ciliatum partly mimics postlarval development
| Autor: | Ethan G. Ross, Nathan A. M. Chrismas, Yousef Touhami, Vengamanaidu Modepalli, Anael Soubigou |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
biology Somatic cell Regeneration (biology) Embryogenesis Sycon ciliatum biology.organism_classification Embryonic stem cell Cell biology Transcriptome 03 medical and health sciences Sponge Multicellular organism 0302 clinical medicine Molecular Biology 030217 neurology & neurosurgery 030304 developmental biology Developmental Biology |
| Zdroj: | Development. |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.193714 |
| Popis: | Somatic cells dissociated from an adult sponge can re-organize and develop into a juvenile-like sponge, a remarkable phenomenon of regeneration. However, the extent to which regeneration recapitulates embryonic developmental pathways has remained enigmatic. We have standardized and established a sponge Sycon ciliatum regeneration protocol from dissociated cells. From the morphological analysis, we demonstrated that dissociated sponge cells follow a series of morphological events resembling postembryonic development. We performed high-throughput sequencing on regenerating samples and compared the data with regular postlarval development. Our comparative transcriptomic analysis illuminates that sponge regeneration is equally as dynamic as embryogenesis. We find that sponge regeneration is orchestrated by recruiting pathways like those utilized in embryonic development. We further demonstrated that sponge regeneration is accompanied by cell death at early stages, revealing the importance of apoptosis in remodelling the primmorphs to initiate re-development. Since sponges are likely to be the first branch of extant multicellular animals, we suggest that this system can be explored to study the genetic features underlying the evolution of multicellularity and regeneration. |
| Databáze: | OpenAIRE |
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