Gallic Acid Inhibits Invasion and Reduces IL-6 Gene Expression, pSTAT3, pERK1/2, and pAKT Cellular Signaling Proteins in Human Prostate Cancer DU-145 Cells
| Autor: | Effat Jafari-Dehkordi, Mahnaz Keloushadi, Esfandiar Heidarian, Keihan Ghatreh-Samani |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Cell signaling Chemistry 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Oncology Gentamicin protection assay 030220 oncology & carcinogenesis Gene expression Cancer research Pharmacology (medical) Radiology Nuclear Medicine and imaging Surgery Viability assay Gallic acid Signal transduction Protein kinase B PI3K/AKT/mTOR pathway |
| Zdroj: | International Journal of Cancer Management. 10 |
| ISSN: | 2538-497X 2538-4422 |
| DOI: | 10.5812/ijcm.9163 |
| Popis: | Background: Prostate cancer (PC) is a malignant disease, which is common in men. Interleukin‑6 (IL-6) mediates the progression of PC through PI3K/AKT, JAK-STAT, and ERK1/2 MAPKs signaling pathways. Gallic acid, a phenolic compound, has anti-oxidant, anti-proliferative, and anti‑tumorigenic properties. Objectives: This study was undertaken to evaluate the effects of gallic acid on DU-145 cell viability, proliferation, invasion, IL-6 gene expression, and the cellular levels of phosphorylated STAT3, ERK1/2, and AKT signaling proteins. Methods: DU-145 cells were treated with gallic acid (0 - 100 µM). The 3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide (MTT) assay was done in order to evaluate the cytotoxicity of gallic acid on DU-145 cells. IL-6 gene expression was investigated, using RT-qPCR. The levels of phosphorylated ERK1/2, AKT, and STAT3 signaling pathways were assessed by Western blotting technic. DU-145 cells invasion were measured by invasion assay test. Results: A significant reduction was observed in viability, proliferation, and invasion of DU-145 cells after treatment with gallic acid. Also, cellular levels of phosphorylated STAT3, ERK1/2, and AKT signaling proteins decreased after 48 hours in a dose-dependent manner by gallic acid. Secretion and gene expression of IL-6 were decreased in DU-145 cells treated with gallic acid. Conclusions: The results of this study showed that gallic acid can lead to a reduction in survival, proliferation, and invasion in DU-145 cell line by reducing protein IL-6 and its gene expression, pSTAT3, pERK1/2, and pAKT signaling protein pathways. Therefore, it seems that gallic acid can be regarded as a potent agent in the treatment of prostate cancer. |
| Databáze: | OpenAIRE |
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