Manipulation of morphological transition in Saccharomyces cerevisiae mutant J19

Autor:	Masayuki Azuma, Jaruwan Maneesri, Hiroshi Ooshima, Koichi Igarashi, Akio Sugihara
Rok vydání:	2005
Předmět:	Genetics Mutation Environmental Engineering biology Transition (genetics) Mutant Saccharomyces cerevisiae Biomedical Engineering Bioengineering biology.organism_classification medicine.disease_cause Phenotype Yeast Cell biology Plasmid medicine Gene Biotechnology
Zdroj:	Biochemical Engineering Journal. 27:72-76
ISSN:	1369-703X
Popis:	Saccharomyces cerevisiae has the potential for morphological differentiation. In order to positively utilize the morphological transition of S. cerevisiae for the separation of cells from culture broth, the cell settling of mutant J19 having pseudohyphal form was examined. Mutant cells settled faster in stationary test tubes than those of the parental strain having yeast-like form. Furthermore, in an attempt to establish a basis for manipulation of the morphological transition between the yeast-like and pseudohyphal forms, a plasmid harboring the SCL 1 gene under the control of the GAL 1 promoter was constructed. The SCL 1 gene complements the morphological phenotype of mutant J19. The plasmid was transformed into mutant J19, and the morphological transition of the transformant by changes in carbon source was examined. It was found that partial manipulation of the morphological transition was possible.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b9c4f28604c98596c36ab684e1b05c3a https://doi.org/10.1016/j.bej.2005.07.001 Zobrazit plný text záznamu Full Text from ScienceDirect