Is the intravascular administration of mesenchymal stem cells safe?
| Autor: | Wenzhong Li, Nan Ma, Dario Furlani, Brigitte Vollmar, Erik Pittermann, Ren-Ke Li, Lee-Lee Ong, Can Yerebakan, Weiwei Wang, Ralf Gaebel, Gustav Steinhoff, Ingeborg Westien, Murat Ugurlucan, Karen Bieback |
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| Rok vydání: | 2009 |
| Předmět: |
Pathology
medicine.medical_specialty business.industry Mesenchymal stem cell Abdominal aorta Ischemia Cell Biology medicine.disease Biochemistry Microcirculation Pulmonary sequestration Transplantation medicine.artery Medicine Arterial blood Cardiology and Cardiovascular Medicine business Intravital microscopy |
| Zdroj: | Microvascular Research. 77:370-376 |
| ISSN: | 0026-2862 |
| DOI: | 10.1016/j.mvr.2009.02.001 |
| Popis: | We investigated the kinetics of human mesenchymal stem cells (MSCs) after intravascular administration into SCID mouse cremaster vasculature by intravital microscopy. MSCs were injected into abdominal aorta through left femoral artery at two different concentrations (1 x 10(6) or 0.2 x 10(6) cell). Arterial blood velocity decrease by 60 and 18% 1 min after high/low dose MSCs injection respectively. The blood microcirculation was interrupted after 174+/-71 and 485+/-81 s. Intravital microscopy observation and histopathologic analysis of cremaster muscles indicated MSCs were entrapped in capillaries in both groups. 40 and 25% animals died of pulmonary embolism respectively in both high and low MSCs dose groups, which was detected by histopathologic analysis of the lungs. Intraarterial MSCs administration may lead to occlusion in the distal vasculature due to their relatively large cell size. Pulmonary sequestration may cause death in small laboratory animals. MSCs should be used cautiously for intravascular transplantation. |
| Databáze: | OpenAIRE |
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