Quantitative evaluation of fluorescencein situ hybridization (FISH) signals in uncultured coelomic cells

Autor:	Steen Kølvraa, G. Bruun-Petersen, D. G. Crüger
Rok vydání:	1997
Předmět:	In situ medicine.medical_specialty Cell type medicine.diagnostic_test Hybridization probe Cytogenetics Obstetrics and Gynecology Biology Fluorescence Molecular biology medicine Coelom Molecular probe Genetics (clinical) Fluorescence in situ hybridization
Zdroj:	Prenatal Diagnosis. 17:635-642
ISSN:	1097-0223 0197-3851
Popis:	Uncultured coelomic cells were hybridized with alpha satellite DNA probes representing chromosomes X, Y, 18, and 13/21 in order to evaluate the distribution of hybridization signals obtained by fluorescence in situ-hybridization (FISH) analysis of this cell type. Cells from 26 samples were hybridized with the X probe and the Y probe was hybridized with cells from 25 of the samples. Cells from 16 and 11 samples were hybridized with an 18 alpha satellite DNA probe and 13/21 alpha satellite probe, respectively. The evaluation demonstrated that FISH with X, Y, 18, and 13/21 alpha satellite DNA probes on uncultured coelomic cells is a reliable technique since the distribution of hybridization signals is comparable to that seen in uncultured amniotic fluid cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b9bf122230235801b20fcb36865a14c2 https://doi.org/10.1002/(sici)1097-0223(199707)17:7<635::aid-pd127>3.0.co Zobrazit plný text záznamu Plný text