Theory and Practice of Using Polycationic Amphiphiles and polymers for In Vitro and In Vivo Gene Transfer

Autor:	B. A. Demeneix, Christophe Benoist, J. S. Rémy, J. P. Behr, Daniel Goula
Rok vydání:	1998
Předmět:	Cell membrane chemistry.chemical_compound medicine.anatomical_structure In vivo Endosome Chemistry Lipofectamine Amphiphile Cationic polymerization medicine Combinatorial chemistry In vitro DNA
Zdroj:	Gene Therapy ISBN: 9783642721625
DOI:	10.1007/978-3-642-72160-1_20
Popis:	The mechanisms underlying the actions of polycationic (as opposed to monocationic) gene transfer vectors is described. Two main types of vectors are examined, polycationic amphiphiles such as DOGS (Transfectam™) and Lipofectamine on the one hand and cationic polymers such as polyethyleneimine on the other hand. The gene transfer performances of these molecules is a function of their DNA condensing capacity, their interactions with anionic proteoglycans of the cell membrane and their capactiy to induce endosome swelling and rupture. The importance of taking into account the overall charge ratio of complexes when carrying out in vitro or in vivogene transfer is emphasized.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b9be91d05d00a5c65d7823427c465cc5 https://doi.org/10.1007/978-3-642-72160-1_20 Zobrazit plný text záznamu