Aversive Training Induces Both Presynaptic and Postsynaptic Suppression inDrosophila
| Autor: | Jianzhi Zeng, Xiaofan Zhang, Ronald L. Davis, Yulong Li, Nathaniel C. Noyes |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell type General Neuroscience Engram Biology Functional imaging 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Odor Postsynaptic potential Mushroom bodies medicine Conditioning Axon Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | The Journal of Neuroscience. 39:9164-9172 |
| ISSN: | 1529-2401 0270-6474 |
| DOI: | 10.1523/jneurosci.1420-19.2019 |
| Popis: | The α′β′ subtype ofDrosophilamushroom body neurons (MBn) is required for memory acquisition, consolidation and early memory retrieval after aversive olfactory conditioning. However,in vivofunctional imaging studies have failed to detect an early forming memory trace in these neurons as reflected by an enhanced G-CaMP signal in response to presentation of the learned odor. Moreover, whether cellular memory traces form early after conditioning in the mushroom body output neurons (MBOn) downstream of the α′β′ MBn remains unknown. Here, we show that aversive olfactory conditioning suppresses the calcium responses to the learned odor in both α′3 and α′2 axon segments of α′β′ MBn and in the dendrites of α′3 MBOn immediately after conditioning using female flies. Notably, the cellular memory traces in both α′3 MBn and α′3 MBOn are short-lived and persist for Areceptor activation. Because activation of the α′3 MBOn drives approach behavior of adult flies, our results demonstrate that aversive conditioning promotes avoidance behavior through suppression of the α′3 MBn–MBOn circuit.SIGNIFICANCE STATEMENTDrosophilalearn to avoid an odor if that odor is repeatedly paired with electric shock. Mushroom body neurons (MBns) are known to be major cell types that mediate this form of aversive conditioning. Here we show that aversive conditioning causes a reduced response to the conditioned odor in an axon branch of one subtype of the MBn for no more than 30 min after conditioning, and in the dendrites of postsynaptic, MB output neurons (MBOns). Because experimenter-induced activation of the MBOn induces approach behavior by the fly, our data support a model that aversive learning promotes avoidance by suppressing the MBn–MBOn synapses that normally promote attraction. |
| Databáze: | OpenAIRE |
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