Simultaneous determination of difloxacin and its primary metabolite sarafloxacin in rabbit plasma
| Autor: | M. A. Bregante, M. A. Garcia, J. J. Aramayona, S. Rueda, C. Solans |
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| Rok vydání: | 2000 |
| Předmět: |
Chromatography
Metabolite fungi Organic Chemistry Clinical Biochemistry Biochemistry High-performance liquid chromatography Fluorescence spectroscopy Analytical Chemistry body regions Standard curve chemistry.chemical_compound Sarafloxacin chemistry medicine Quantitative analysis (chemistry) Difloxacin Antibacterial agent medicine.drug |
| Zdroj: | Chromatographia. 51:487-490 |
| ISSN: | 1612-1112 0009-5893 |
| DOI: | 10.1007/bf02490490 |
| Popis: | An HPLC method with fluorescence detection is presented for the analysis of difloxacin (DIF) and sarafloxacin (SAR) in rabbit plasma using norfloxacin (NOR) as internal standard (Figure 1). Plasma sample preparations were carried out by adding phosphate buffer (pH 7.4, 0.1 M), followed by extraction with trichloromethane. Fluoroquinolones were separated on a reversed-phase column using an aqueous phosphate solution-acetonitrile (82:18) mobile phase. The concentrations of NOR, SAR and DIF eluting off the column, with retention times of 2.16, 5.60 and 6.20, respectively, were monitored by fluorescence detection atλ ex 338 andλ em 425 nm. The quantitation limit was 12 ng mL−1 for SAR and DIF. Standard curves were linearly related to concentration in the range from 1 to 1500 ng mL−1. Recovery was determined as 76% and 70% for SAR and DIF, respectively. Inter-and intraassay coefficients of variation were less than 6% for all compounds. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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