Analytical HPLC Method Validation of Amiloride and Its Pharmacokinetic Study in Humans

Autor: Chang-Ik Choi, Seok-Yong Lee, Young Seo Park, Mi-Jeong Kim, Chang-Seon Myung, Jung-Woo Bae, Joon-Ho Sa, Yee Song, Choon-Gon Jang
Rok vydání: 2008
Předmět:
Zdroj: Journal of Liquid Chromatography & Related Technologies. 31:2455-2466
ISSN: 1520-572X
1082-6076
DOI: 10.1080/10826070802319693
Popis: This study was aimed to validate a reliable analytical method for the pharmacokinetic study of amiloride in human plasma by a high performance liquid chromatography (HPLC) system with UV detection. Triamterene was used as an internal standard. After extraction with ethylacetate, the supernatant was evaporated. Then, the residue was reconstituted and an aliquot was injected onto the HPLC system. Separation was performed on a Capcell Pak C18 UG120 column (4.6 mm × 150 mm, 5 µm particles) with a mobile phase of 12% acetonitrile containing 0.4% glacial acetic acid and UV detection at a wavelength of 360 nm. The intra- and inter-day precision expressed as the relative standard deviation was less than 15%. The flow rate of mobile phase was 1 mL/min and the retention time of amiloride and internal standard, triamterene, was found to be 3.06 and 8.80 min, respectively. The lower limit of quantification (LOQ) was 0.2 ng/mL of amiloride using 1 mL of plasma. The calibration curve was linear in the concentr...
Databáze: OpenAIRE
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