| Popis: |
Leukocyte adhesion to endothelial cells is known to be mediated through adhesion molecules, such as CD11b/CD18, a member of LeuCAM (Leukocyte Cellular Adhesion Molecule) family. As previously described, we confirmed that CD11b/CD18 expression was enhanced on monocytes of insulin-dependent diabetic patients with proliferative retinopathy. We report here that pentoxifylline incubation induced a significant decrease in CD1lb/CD18 expression of normal monocytes. Similarly, pentoxifylline pretreatment inhibited the augmentation of CD1lb/CD18 expression on monocytes stimulated with A23187, f-MLP and C5a. The adhesion of pentoxifylline treated monocytes to fibronectin and plasma coated surfaces was decreased.The adhesion of monocytes from diabetic patients was increased on human endothelial cells (HEC) both with and without the stimulation of IL-1. The significant inhibitory effect of pentoxifylline on the adhesion of monocytes to endothelial cells was more apparent when HEC were not stimulated with IL-1. These results indicated that in in-vitro conditions, pentoxifylline inhibited the adhesion of monocytes to various surfaces and decreased the expression of CD1lb/CD18 on monocytes. |
