Manganese (II) oxidation by microperoxidase-11 in hydrophilic organic media
| Autor: | Mari Kabuto, Masafumi Oyadomari, Hiroyuki Wariishi, Hiroo Tanaka |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Environmental Engineering
Aqueous solution biology Chemistry Depolymerization Inorganic chemistry Biomedical Engineering chemistry.chemical_element Bioengineering Manganese biology.organism_classification Catalysis chemistry.chemical_compound Catalytic cycle Manganese peroxidase Phanerochaete Methanol Biotechnology |
| Zdroj: | Biochemical Engineering Journal. 15:159-164 |
| ISSN: | 1369-703X |
| DOI: | 10.1016/s1369-703x(02)00184-5 |
| Popis: | Microperoxidase-11 (MP-11) derived from horse heart cytochrome c was utilized as a peroxidative catalyst. Mn II -oxidation activity of MP-11 was demonstrated for the first time. Although the activity of MP-11 was low compared to manganese peroxidase (MnP) from Phanerochaete chrysosporium in aqueous system, it can be utilized in water-miscible organic solvents if at least 5% water exists. Mn II -oxidation by MP-11 was optimized at higher pH compared to MnP, probably because of the lack of distal His acting as a proton acceptor during the catalytic cycle. Finally, the depolymerization of lignin was also achieved in 90% methanol using MP-11 in the presence of H 2 O 2 and Mn II . |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect