Higher SARS-CoV-2 detection of oropharyngeal compared with nasopharyngeal or saliva specimen for molecular testing: a multicentre randomised comparative accuracy study
| Autor: | Tobias Todsen, Martin G Tolsgaard, Thomas Benfield, Fredrik Folke, Kathrine K Jakobsen, Niels Tobias Gredal, Annette K Ersbøll, Christian von Buchwald, Nikolai Kirkby |
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| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Thorax. :thorax-2022 |
| ISSN: | 1468-3296 0040-6376 |
| Popis: | BackgroundTesting is critical for detecting SARS-CoV-2 infection, but the best sampling method remains unclear.ObjectivesTo determine whether nasopharyngeal swab (NPS), oropharyngeal swab (OPS) or saliva specimen collection has the highest detection rate for SARS-CoV-2 molecular testing.MethodsWe conducted a randomised clinical trial at two COVID-19 outpatient test centres where NPS, OPS and saliva specimens were collected by healthcare workers in different orders for reverse transcriptase PCR testing. The SARS-CoV-2 detection rate was calculated as the number positive by a specific sampling method divided by the number in which any of the three sampling methods was positive. As secondary outcomes, test-related discomfort was measured with an 11-point numeric scale and cost-effectiveness was calculated.ResultsAmong 23 102 adults completing the trial, 381 (1.65%) were SARS-CoV-2 positive. The SARS-CoV-2 detection rate was higher for OPSs, 78.7% (95% CI 74.3 to 82.7), compared with NPSs, 72.7% (95% CI 67.9 to 77.1) (p=0.049) and compared with saliva sampling, 61.9% (95% CI 56.9 to 66.8) (pConclusionsOPSs were associated with higher SARS-CoV-2 detection and lower test-related discomfort than NPSs for SARS-CoV-2 testing. Saliva sampling had the lowest SARS-CoV-2 detection but was the least costly strategy for mass testing.Trial registration numberNCT04715607. |
| Databáze: | OpenAIRE |
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