| Popis: |
This paper presents a method combined of HPLC and DAD in detecting the total nitrogen index in urban waste water and studies the digestion condition of the sample pretreatment. The result shows that after pressurized digestion and adjusting pH to around 7.0 with phosphoric acid buffer solution, the sample can be applied chromatographic analysis. The chromatographic analysis is conducted with phosphoric acid buffer solution-acetonitrile as the mobile phase, flow velocity 0.8mL.min-1, column temperature 30 °C, intake-sample volume 10 μL,with ODS as the stationary phase, and the sample is detected with wavelength of 208nm. The linearly dependent coefficient of standard curve is 0.9971, the linearity range is 0.10-30.0mg·L-1, relative standard deviation (RSD) is less than 12%, the recycle rate is 107-159%,detection limit (S/N=2.4) reaches 0.05ng. |
