Biomolecule composition and draft genome of a novel, high-lipid producing Scenedesmaceae microalga
| Autor: | Prateek Shetty, Ervin Balázs, Vince Ördög, Vilmos Soós, Eszter Badics, Gergely Maróti, Norbert Incze, Péter Bálint |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Genetics 0303 health sciences Nuclear gene biology biology.organism_classification 01 natural sciences Genome DNA sequencing Transcriptome Chloroplast 03 medical and health sciences Lipid biosynthesis Scenedesmaceae Agronomy and Crop Science Gene 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | Algal Research. 54:102181 |
| ISSN: | 2211-9264 |
| DOI: | 10.1016/j.algal.2020.102181 |
| Popis: | Lipid biosynthesis in microalgae can be stimulated by cultivation in low nitrogen medium. MACC-401 was isolated from the soil surface in Tres Marias (MG-Brazil). The strain shows the morphological characteristics of the Scenedesmaceae green algae. The daily biomass and lipid production of MACC-401 is remarkable, 0.36 g L−1 and 110 mg L−1, respectively. Exploration of the genetic background of this promising strain not only allows the utilization of this species for industrial-scale lipid production, but also provides genetic targets to select lipid-producing strains from microalgae collections. We conducted physiological experiments by cultivating MACC-401 in complete and N-limited media and performed genome sequencing as well as transcriptome analysis. The estimated nuclear genome size of MACC-401 is 99.503 Mbp and the chloroplast genome is 0.15 Mbp. The phylogenetic analysis confirmed that the MACC-401 belongs to the Scenedesmaceae family, and represents a genetically distinct accession in this family. A basic comparative transcriptome analysis resulted in the identification of N-starvation responsive genes, which could serve as markers to monitor the onset of lipid accumulation in algal cultures. |
| Databáze: | OpenAIRE |
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