Role of diagnostic intracytoplasmic sperm injection (ICSI) in the management of genetically determined zona pellucida-free oocytes during in vitro fertilization: a case report
| Autor: | Hadiya Brasha, Mohamed Fawzy, Ali Hellani, Ahmad M. Metwalley, Sandro C. Esteves, Omaima Khamiss, Nabeel Brasha, Manal El Hamshary |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
030219 obstetrics & reproductive medicine In vitro fertilisation urogenital system business.industry medicine.medical_treatment Natural cycle Embryogenesis Cell Biology Oocyte Intracytoplasmic sperm injection Andrology 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure embryonic structures medicine business Zona pellucida reproductive and urinary physiology Exome sequencing 030304 developmental biology Developmental Biology Unexplained infertility |
| Zdroj: | Zygote. 28:519-523 |
| ISSN: | 1469-8730 0967-1994 |
| DOI: | 10.1017/s0967199420000441 |
| Popis: | Purpose:To report the utilization of diagnostic intracytoplasmic sperm injection (D-ICSI), an ICSI cycle performed in the natural cycle, to obtain information about embryo development potential after sperm injection into zona pellucida (ZP)-free oocytes.Materials and Methods:We report the case of a couple with primary unexplained infertility with a history of previous failed, in vitro fertilization intracytoplasmic sperm injection (IVF-ICSI) cycles characterized by the presence of ZP-free oocytes. Whole exome sequencing (WES) was carried out to analyse the possible genetic basis of oocyte abnormality.Results:Diagnostic ICSI provided information about the embryo development potential from ZP-free oocytes and allowed better planning of the subsequent ICSI cycle. WES revealed that the absence of ZP was likely to be due to a new (ZP1) mutation. The subsequent ICSI cycle resulted in the delivery of a healthy baby.Discussion:To the best of our knowledge, our report is the first to describe the use of D-ICSI to determine the feasibility of embryo development and implantation in a patient with ZP1 mutation, resulting in the subsequent delivery of a healthy baby. We used ‘diagnostic’ ICSI in the normal menstrual cycle to explore the feasibility of embryo development after sperm injection into ZP-free oocytes. Our results may expand the spectrum of diagnostic procedures associated with unexplained infertility. |
| Databáze: | OpenAIRE |
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