Autor:	L. Nsapato, K.K. Nkongolo
Rok vydání:	2003
Předmět:	Genetics Genetic diversity Phylogenetic tree food and beverages Plant Science Biology Analysis of molecular variance RAPD Genetic distance Genetic marker Genetic variation Genetic variability Agronomy and Crop Science Ecology Evolution Behavior and Systematics
Zdroj:	Genetic Resources and Crop Evolution. 50:149-156
ISSN:	0925-9864
DOI:	10.1023/a:1022996211164
Popis:	Genetic variation within and among several Sorghum populations from different agroecological zones in Malawi were investigated using random amplified polymorphic markers (RAPDs). DNA samples from individual plants were analyzed using 35 oligonucleotides of random sequence. Twenty five of these primers allowed amplifications of random polymorphic (RAPD) loci. Overall, 52% of the scored loci were polymorphic. Every accession was genetically distinct. The analysis of molecular variance revealed that the within-region (among accessions) variations accounted for 96.43% of the total molecular variance. Observed variations in allelic frequency was not related to agroecological differences. The degree of band sharing was used to evaluate genetic distance between accessions and to construct a phylogenetic tree. Further analysis revealed that the sorghum accessions analyzed were genetically close despite considerable phenotypic diversity within and among them. It is suggested that all the sorghum landraces currently available in Malawi should be conserved both ex situ and in situ to maintain the current level of genetic diversity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b908ba14552483a5701981bf68456b7c https://doi.org/10.1023/a:1022996211164 Zobrazit plný text záznamu Full text from SpringerLink